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4.1.2 EXTRACTION TIME PROFILES Extraction time profiles for CBZ using C18 coatings of varying thickness (n=3 fibres) were constructed in order to determine the time required to reach equilibrium. As shown in Figure 3, using static conditions the equilibrium was reached in 60 minutes (15 μm coating) and 300 minutes (30 μm coating). These times are too long to perform equilibrium in vivo SPME sampling thus necessitating the use of kinetic calibration method. For in vitro testing, agitation can be used in order to significantly decrease the equilibration times for these fibres as shown in Figure 4. Using 2400 rpm vortex agitation equilibrium was reached in 60 minutes for C18 and C16-amide (45 μm) coatings (n=3 fibres).
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